Recombinant GH5_8 mannanase from Hungateiclostridium cellulolyticum: biochemical and structural characteristics and applications for mannooligosaccharides production from spent coffee grounds.
SILVA, Guilherme Romualdo; CAPETTI, Caio Cesar de Mello; DABUL, Andrei Nicoli Gebieluca; LIBERATO, Marcelo Vizoná; CURVELO, Antonio Aprigio da Silva; PELLEGRINI, Vanessa Rodrigues; POLIKARPOV, Igor.
SILVA, Guilherme Romualdo; CAPETTI, Caio Cesar de Mello; DABUL, Andrei Nicoli Gebieluca; LIBERATO, Marcelo Vizoná; CURVELO, Antonio Aprigio da Silva; PELLEGRINI, Vanessa Rodrigues; POLIKARPOV, Igor.
Abstract: Abundant agro-industrial residues, spent coffee grounds (SCG), are rich in polysaccharides, notably mannan, rendering them a promising substrate for the production of mannooligosaccharides (MOS) with potential nutraceutical applications. This work reports cloning, heterologous expression in E. coli Arctic Express and biochemical characterization of Hungateiclostridium cellulolyticum mannanase from GH5 family (HcGH5_8) and its application for producing MOS from pretreated SCG. The enzyme displayed optimal activity at pH 7.0 and 50 °C, with a specific activity of 687 ± 53 U/mg against konjac glucomannan under optimal conditions. Furthermore, hydrolysis of the SCG resulted in the release of mannobiose (M2), mannotriose (M3), and mannotetraose (M4) as the primary hydrolytic products. The impacts of different hydrothermal pretreatment conditions on the yields of the enzymatically-produced MOS were investigated.
@article={003238954,author = {SILVA, Guilherme Romualdo; CAPETTI, Caio Cesar de Mello; DABUL, Andrei Nicoli Gebieluca; LIBERATO, Marcelo Vizoná; CURVELO, Antonio Aprigio da Silva; PELLEGRINI, Vanessa Rodrigues; POLIKARPOV, Igor.},title={Recombinant GH5_8 mannanase from Hungateiclostridium cellulolyticum: biochemical and structural characteristics and applications for mannooligosaccharides production from spent coffee grounds},journal={Food and Bioproducts Processing},note={v. 151, p. 44-53},year={2025}}