Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by using Giemsa dye as a photosensitizer.
CAIRES, Cynthia Suzyelen Albuquerque; LIMA, Alessandra Ramos; LIMA, Thalíta Hellen Nunes; SILVA, Cícera Maria da; ARAUJO, Leandro Oliveira; AGUILERA, Laís Fernandes; NASCIMENTO, Valter Aragão do; CAIRES, Anderson Rodrigues Lima; OLIVEIRA, Samuel Leite de.
CAIRES, Cynthia Suzyelen Albuquerque; LIMA, Alessandra Ramos; LIMA, Thalíta Hellen Nunes; SILVA, Cícera Maria da; ARAUJO, Leandro Oliveira; AGUILERA, Laís Fernandes; NASCIMENTO, Valter Aragão do; CAIRES, Anderson Rodrigues Lima; OLIVEIRA, Samuel Leite de.
Abstract: The rise of antibiotic-resistant bacteria calls for innovative approaches to combat multidrug-resistant strains. Here, the potential of the standard histological stain, Giemsa, to act as a photosensitizer (PS) for antimicrobial photodynamic inactivation (aPDI) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains is reported. Bioassays were performed using various Giemsa concentrations (ranging from 0.0 to 20.0 µM) under 625 nm illumination at a light dose of 30 J cm-2. Remarkably, Giemsa completely inhibited the growth of MSSA and MRSA bacterial colonies for concentrations at 10 µM and higher but exhibited no inhibitory effect without light exposure. Partition coefficient analysis revealed Giemsa's affinity for membranes. Furthermore, we quantified the production of reactive oxygen species (ROS) and singlet oxygen (1O2) to elucidate the aPDI mechanisms underlying bacterial inactivation mediated by Giemsa. These findings highlight Giemsa stain's potential as a PS in aPDI for targeting multidrug-resistant bacteria.
@article={003182268,author = {CAIRES, Cynthia Suzyelen Albuquerque; LIMA, Alessandra Ramos; LIMA, Thalíta Hellen Nunes; SILVA, Cícera Maria da; ARAUJO, Leandro Oliveira; AGUILERA, Laís Fernandes; NASCIMENTO, Valter Aragão do; CAIRES, Anderson Rodrigues Lima; OLIVEIRA, Samuel Leite de.},title={Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by using Giemsa dye as a photosensitizer},journal={Photodiagnosis and Photodynamic Therapy},note={v. 45, p. 103952-1-103952-7},year={2024}}