Design of a low-cost and disposable paper-based immunosensor for the rapid and sensitive detection of aflatoxin B1.
MIGLIORINI, Fernanda L.; SANTOS, Danilo M. dos; SOARES, Andrey Coatrini; MATTOSO, Luiz H. C.; OLIVEIRA JUNIOR, Osvaldo Novais de; CORREA, Daniel S.
MIGLIORINI, Fernanda L.; SANTOS, Danilo M. dos; SOARES, Andrey Coatrini; MATTOSO, Luiz H. C.; OLIVEIRA JUNIOR, Osvaldo Novais de; CORREA, Daniel S.
Abstract: We report a paper-based electrochemical immunosensor made with sustainable materials to detect aflatoxin B1 (AFB1), a highly toxic, carcinogenic mycotoxin found in food. The immunosensor was prepared with a waterproof paper substrate and low-cost graphite-based conductive ink through a simple cut-printing method. The working electrode was functionalized with a drop-cast film of multiwalled carbon nanotubes (MWCNT)/chitosan on which a layer of anti-AFB1 monoclonal antibodies was immobilized covalently. The architecture of the immunosensor was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and electrochemical impedance spectroscopy (EIS), including the e ective immobilization of the active layer of anti-AFB1. With EIS as the principle of detection, the immunosensor could detect AFB1 in the range from 1 to 30 ng mL-1, and detection limit of 0.62 ng mL-1. This sensitivity is su cient to detect AFB1 in food according to regulatory agencies. The immunosensor exhibited good repeatability, reproducibility, stability, and selectivity in experiments with a possible interferent. Furthermore, detection of AFB1 in maize flour samples yielded recovery of 97-99%, in a demonstration of the possible use of the paper-based immunosensor to detect AFB1 using extraction solutions from food samples.
@article={003006652,author = {MIGLIORINI, Fernanda L.; SANTOS, Danilo M. dos; SOARES, Andrey Coatrini; MATTOSO, Luiz H. C.; OLIVEIRA JUNIOR, Osvaldo Novais de; CORREA, Daniel S.},title={Design of a low-cost and disposable paper-based immunosensor for the rapid and sensitive detection of aflatoxin B1},journal={Chemosensors},note={v. 8, n. 3, p. 87-1-87-14},year={2020}}