Improved antibody loading on self-assembled graphene oxide films for using in surface plasmon resonance immunosensors.
MIYAZAKI, Celina Massumi; CAMILO, Douglas Eleutério; SHIMIZU, Flavio Makoto; FERREIRA, Marystela.
MIYAZAKI, Celina Massumi; CAMILO, Douglas Eleutério; SHIMIZU, Flavio Makoto; FERREIRA, Marystela.
 Abstract: Immunoassays are important for the diagnosis of many diseases. Efficient methods for the immobilization of the biorecognition elements is a relevant subject because the antibody loading is directly related to the detection range and sensitivity. Here different self-assembled films were explored to take advantage of polar oxygenated groups from graphene oxide (GO) structure. Surface plasmon resonance (SPR) sensors were modified with different GO self-assembled films and compared to the well-established thiol-functionalization with 11-MUA (11- mercaptoundecanoic acid). Self-assembled cysteamine/GO functionalized sensor (Cys-GOSA) provided the highest protein loading (7.66×10 -12 mol cm-2 against 1.29×10 -11 mol cm-2 for the traditional thiolfunctionalization). The Langmuir-Blodgett (LB) films of GO were composed of aggregates and empty spaces, which hindered the antibody anchoring (around 6.38×10 -12 to 3.45×10 -12 mol cm-2). The SPR response of the Cys-GOSA-modified sensor to the 4.0 ng mL-1 PSA was 125% higher than the thiol-functionalized sensor at the same concentration. This result indicates a simple and promising surface modification strategy for many important applications, not limited to SPR sensors, but for all immunoreaction-based assays as enzyme-linked immunosorbent assay (ELISA), electrochemical (e.g. amperometric and impedimetric) and quartz crystal microbalance (QCM) based sensors. | |
| Applied Surface Science |
| v. 490, p. 502-509 - Ano: 2019 |
| Fator de Impacto: 5,155 |
| http://dx.doi.org/10.1016/j.apsusc.2019.06.095 |  @article={002951031,author = {MIYAZAKI, Celina Massumi; CAMILO, Douglas Eleutério; SHIMIZU, Flavio Makoto; FERREIRA, Marystela.},title={Improved antibody loading on self-assembled graphene oxide films for using in surface plasmon resonance immunosensors},journal={Applied Surface Science},note={v. 490, p. 502-509},year={2019}} |