Polyhexamethylene biguanide nanoparticles inhibit biofilm formation by mastitis-causing Staphylococcus aureus.
LEITE, Renata de Freitas; GARCIA, Breno Luis Nery; BARBOSA, Kristian da Silva; MITSUNAGA, Thatiane Mendes; FIDELIS, Carlos Eduardo; DIAS, Bruna Juliana Moreira; MIRANDA, Renata Rank; ZUCOLOTTO, Valtencir; GOOD, Liam; SANTOS, Marcos Veiga dos.
LEITE, Renata de Freitas; GARCIA, Breno Luis Nery; BARBOSA, Kristian da Silva; MITSUNAGA, Thatiane Mendes; FIDELIS, Carlos Eduardo; DIAS, Bruna Juliana Moreira; MIRANDA, Renata Rank; ZUCOLOTTO, Valtencir; GOOD, Liam; SANTOS, Marcos Veiga dos.
Abstract: This manuscript addresses the persistent challenge of Staphylococcus aureus, a primary agent of bovine mastitis that compromises animal welfare and results in substantial economic losses in dairy production. The difficulty in treating S. aureus lies in its resistance to multiple antibiotics and its capacity to form resilient biofilms within mammary gland cells, which hampers the effectiveness of conventional therapies. In response, the study evaluates a novel strategy involving polyhexamethylene biguanide (PHMB) nanoparticles, known for their antimicrobial properties. Two main experiments were conducted. The first assessed the cytotoxicity of PHMB nanoparticles and their ability to disrupt S. aureus biofilms in vitro. The findings reveal that these nanoparticles were well tolerated by bovine mammary cells and significantly inhibited biofilm formation at effective concentrations. The second experiment compared PHMB nanoparticles to commonly used post-milking disinfectants. While the nanoparticles did not outperform traditional treatments, they still reduced bacterial load, with results possibly influenced by limited exposure time during application. Despite this, the study highlights the potential of PHMB nanoparticles as an alternative approach for controlling mastitis caused by S. aureus, especially in biofilm-associated infections. This innovative method may contribute to improved udder health and enhanced productivity in the dairy industry, warranting further investigation under practical conditions.
@article={003251635,author = {LEITE, Renata de Freitas; GARCIA, Breno Luis Nery; BARBOSA, Kristian da Silva; MITSUNAGA, Thatiane Mendes; FIDELIS, Carlos Eduardo; DIAS, Bruna Juliana Moreira; MIRANDA, Renata Rank; ZUCOLOTTO, Valtencir; GOOD, Liam; SANTOS, Marcos Veiga dos.},title={Polyhexamethylene biguanide nanoparticles inhibit biofilm formation by mastitis-causing Staphylococcus aureus},journal={Veterinary Sciences},note={v. 12, n. 5, p. 507-1-507-23},year={2025}}