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Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase.
SEPULCHRO, Ana Gabriela Veiga; PELLEGRINI, Vanessa de Oliveira Arnoldi; BRIGANTI, Lorenzo; ARAÚJO, Evandro Ares de; ARAÚJO, Simara Semiramis; POLIKARPOV, Igor.
Abstract: Enzymatic transformation of xylans into renewable fuels and value-added products is mediated by xylanases. Here we describe the biochemical and X-ray structural characterization of Thermobacillus composti GH10 xylanase (TcXyn10A) at 2.1 Å resolution aiming to unravel details of its recognition of glucurono- and arabinoxylan at a molecular level. TcXyn10A improves the efficiency of pretreated lignocellulosic biomass hydrolysis by a commercial enzyme cocktail causing a 15.35 % increase in xylose release and 4.38 % glucose release after 24 h of reaction. The enzyme releases predominantly xylobiose and xylotriose, as well as MeGlcA3 × 3 (from beechwood glucuronoxylan) and a range of decorated xylooligosaccharides (XOS) from rye arabinoxylan, with Ara2 × 2 being the major product. The enzyme liberates XOS with the yields of 29.09 % for beechwood glucuronoxylan and 16.98 % for rye arabinoxylan. Finally, TcXyn10A has a high thermal stability, halotolerance, and resistance to ethanol, biochemical properties that can be desirable for a number of industrial applications.
Carbohydrate Polymers
v. 247, p. 116714-1-116714-14 - Ano: 2020
Fator de Impacto: 7,182
    @article={003000125,author = {SEPULCHRO, Ana Gabriela Veiga; PELLEGRINI, Vanessa de Oliveira Arnoldi; BRIGANTI, Lorenzo; ARAÚJO, Evandro Ares de; ARAÚJO, Simara Semiramis; POLIKARPOV, Igor.},title={Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase},journal={Carbohydrate Polymers},note={v. 247, p. 116714-1-116714-14},year={2020}}