Designing silver nanoparticles for detecting Levodopa (3,4-dihydroxyphenylalanine, L-Dopa) using surface-enhanced raman scattering (SERS).
RUBIRA, Rafael Jesus Gonçalves; CAMACHO, Sabrina Alessio; MARTIN, Cibely Silva; MEJÍA-SALAZAR, Jorge Ricardo; GÓMEZ, Faustino Reyes; SILVA, Robson Rosa da; OLIVEIRA JUNIOR, Osvaldo Novais de; ALESSIO, Priscila; CONSTANTINO, Carlos José Leopoldo.
RUBIRA, Rafael Jesus Gonçalves; CAMACHO, Sabrina Alessio; MARTIN, Cibely Silva; MEJÍA-SALAZAR, Jorge Ricardo; GÓMEZ, Faustino Reyes; SILVA, Robson Rosa da; OLIVEIRA JUNIOR, Osvaldo Novais de; ALESSIO, Priscila; CONSTANTINO, Carlos José Leopoldo.
Abstract: Detection of the drug Levodopa (3,4-dihydroxyphenylalanine, L-Dopa) is essential for the medical treatment of several neural disorders, including Parkinson's disease. In this paper, we employed surface-enhanced Raman scattering (SERS) with three shapes of silver nanoparticles (nanostars, AgNS; nanospheres, AgNP; and nanoplates, AgNPL) to detect L-Dopa in the nanoparticle dispersions. The sensitivity of the L-Dopa SERS signal depended on both nanoparticle shape and L-Dopa concentration. The adsorption mechanisms of L-Dopa on the nanoparticles inferred from a detailed analysis of the Raman spectra allowed us to determine the chemical groups involved. For instance, at concentrations below/equivalent to the limit found in human plasma (between 10 -7-10 -8 mol/L), L-Dopa adsorbs on AgNP through its ring, while at 10 -5-10 -6 mol/L adsorption is driven by the amino group. At even higher concentrations, above 10 -4 mol/L, L-Dopa polymerization predominates. Therefore, our results show that adsorption depends on both the type of Ag nanoparticles (shape and chemical groups surrounding the Ag surface) and the L-Dopa concentration. The overall strategy based on SERS is a step forward to the design of nanostructures to detect analytes of clinical interest with high specificity and at varied concentration ranges.
@article={002981807,author = {RUBIRA, Rafael Jesus Gonçalves; CAMACHO, Sabrina Alessio; MARTIN, Cibely Silva; MEJÍA-SALAZAR, Jorge Ricardo; GÓMEZ, Faustino Reyes; SILVA, Robson Rosa da; OLIVEIRA JUNIOR, Osvaldo Novais de; ALESSIO, Priscila; CONSTANTINO, Carlos José Leopoldo.},title={Designing silver nanoparticles for detecting Levodopa (3,4-dihydroxyphenylalanine, L-Dopa) using surface-enhanced raman scattering (SERS)},journal={Sensors},note={v. 20, n. 1, p. 15-1-15-18},year={2020}}