Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application.
VELASCO, Josman; OLIVA, Bianca; MULINARI, Evandro José; QUINTERO, Leidy Patricia; LIMA, Awana da Silva; GONÇALVES, Aline Larissa; GONÇALVES, Thiago Augusto; DAMASIO, André; SQUINA, Fabio Marcio; MILAGRES, Adriane Maria Ferreira; ABDELLA, Asmaa; WILKINS, Mark R.; SEGATO, Fernando.
VELASCO, Josman; OLIVA, Bianca; MULINARI, Evandro José; QUINTERO, Leidy Patricia; LIMA, Awana da Silva; GONÇALVES, Aline Larissa; GONÇALVES, Thiago Augusto; DAMASIO, André; SQUINA, Fabio Marcio; MILAGRES, Adriane Maria Ferreira; ABDELLA, Asmaa; WILKINS, Mark R.; SEGATO, Fernando.
Abstract: Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-ß-1,4-xylanase from Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed this AFUMN-GH10 does not contain a carbohydrate-binding module. The enzyme retained its activity in a pH range from 4.5 to 7.0, with an optimal temperature at 60 °C. AFUMN-GH10 showed the highest activity in beechwood xylan. The mode of action of AFUMN-GH10 was investigated by hydrolysis of APTS-labeled xylohexaose, which resulted in xylotriose and xylobiose as the main products. AFUMN-GH10 released 27% of residual xylan from hydrothermally-pretreated corn stover and 14% of residual xylan from hydrothermally-pretreated sugarcane bagasse. The results showed that environmentally friendly pretreatment followed by enzymatic hydrolysis with AFUMN-GH10 in low concentration is a suitable method to remove part of residual and recalcitrant hemicellulose from biomass.
@article={002980312,author = {VELASCO, Josman; OLIVA, Bianca; MULINARI, Evandro José; QUINTERO, Leidy Patricia; LIMA, Awana da Silva; GONÇALVES, Aline Larissa; GONÇALVES, Thiago Augusto; DAMASIO, André; SQUINA, Fabio Marcio; MILAGRES, Adriane Maria Ferreira; ABDELLA, Asmaa; WILKINS, Mark R.; SEGATO, Fernando.},title={Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application},journal={Biotechnology Reports},note={v. 24, p. e00382-1-e00382-10},year={2019}}