Copper nanoparticles stabilized with cashew gum: antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line.
AMORIM, Adriany; MAFUD, Ana Carolina; NOGUEIRA, Silvania; RAMOS-JESUS, Joilson; ARAÚJO, Alyne Rodrigues de; PLÁCIDO, Alexandra; BRITO NETA, Maria; ALVES, Michel Muálem Moraes; CARVALHO, Fernando Aécio Amorim; ARCANJO, Daniel Dias Rufino; BRAUN, Sacha; LÓPEZ, Marta Sánchez-Paniagua; LÓPEZ-RUIZ, Beatriz; DELERUE-MATOS, Cristina; MASCARENHAS, Yvonne Primerano; SILVA, Durcilene; EATON, Peter; LEITE, José Roberto de Souza de Almeida.
AMORIM, Adriany; MAFUD, Ana Carolina; NOGUEIRA, Silvania; RAMOS-JESUS, Joilson; ARAÚJO, Alyne Rodrigues de; PLÁCIDO, Alexandra; BRITO NETA, Maria; ALVES, Michel Muálem Moraes; CARVALHO, Fernando Aécio Amorim; ARCANJO, Daniel Dias Rufino; BRAUN, Sacha; LÓPEZ, Marta Sánchez-Paniagua; LÓPEZ-RUIZ, Beatriz; DELERUE-MATOS, Cristina; MASCARENHAS, Yvonne Primerano; SILVA, Durcilene; EATON, Peter; LEITE, José Roberto de Souza de Almeida.
 Abstract: Copper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, using the cashew gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, and x-ray diffraction were used to characterize the nanoparticles (CG-CuNPs), and copper was quantified by electrochemical measurement. The UV-vis spectra of the CG-CuNPs confirmed the formation of nanoparticles by appearance of a surface plasmon band at 580 nm after 24 h of reaction. The Fourier-transform infrared spectrum of CG-CuNPs showed the peak at 1704 cm 1 from cashew gum, confirming the presence of the gum in the nanoparticles. The average size of CG-CuNPs by dynamic light scattering and atomic force microscopy was around 10 nm, indicating small, approximately spherical particles. Antimicrobial assays showed that CG-CuNPs had activity against Staphylococcus aureus ATCC 29213 with a minimal inhibitory concentration of 0.64 mM. The cytotoxicity assay on BALB/c murine macrophages showed lower cytotoxic effects for CG-CuNPs than CuSO4 5H2O. Viability cell assays for CG-CuNPs at (0.250 mM) inhibited by 70% the growth of 4T1 LUC (4T1 mouse mammary tumor cell line) and NIH 3T3 cells (murine fibroblast cells) over a 24-h period. Therefore, CG-CuNPs can be used as an antimicrobial agent with lower cytotoxic effects than the CuSO4 5H2O precursor. | |
| Journal of Biomaterials Applications |
| v. 34, n. 2, p. 188-197 - Ano: 2019 |
| Fator de Impacto: 2,442 |
| https://doi.org/10.1177/0885328219845964 |  @article={002954897,author = {AMORIM, Adriany; MAFUD, Ana Carolina; NOGUEIRA, Silvania; RAMOS-JESUS, Joilson; ARAÚJO, Alyne Rodrigues de; PLÁCIDO, Alexandra; BRITO NETA, Maria; ALVES, Michel Muálem Moraes; CARVALHO, Fernando Aécio Amorim; ARCANJO, Daniel Dias Rufino; BRAUN, Sacha; LÓPEZ, Marta Sánchez-Paniagua; LÓPEZ-RUIZ, Beatriz; DELERUE-MATOS, Cristina; MASCARENHAS, Yvonne Primerano; SILVA, Durcilene; EATON, Peter; LEITE, José Roberto de Souza de Almeida.},title={Copper nanoparticles stabilized with cashew gum: antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line},journal={Journal of Biomaterials Applications},note={v. 34, n. 2, p. 188-197},year={2019}} |