Crystal structure of the GDP-bound GTPase era from Staphylococcus aureus.
KLOCHKOVA, Evelina; BIKTIMIROV, Artem; ISLAMOV, Daut; BELUSOUV, Anatolii Sergeevich; VALIDOV, Shamil; YUSUPOV, Marat; USACHEV, Konstantin.
KLOCHKOVA, Evelina; BIKTIMIROV, Artem; ISLAMOV, Daut; BELUSOUV, Anatolii Sergeevich; VALIDOV, Shamil; YUSUPOV, Marat; USACHEV, Konstantin.
Abstract: GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.76 Å resolution crystal structure of Staphylococcus aureus Era in complex with GDP is presented. Structural comparison with other GTP-bound and GDP-bound homologous proteins, GTPase domain and the KH domain revealed a mutual orientation in S. aureus which has not been described before. The GDP-bound Era structure presented here will facilitate efforts to elucidate its interactions with its regulators and lay the foundation for a structure-based search for specific inhibitors.
@article={003236767,author = {KLOCHKOVA, Evelina; BIKTIMIROV, Artem; ISLAMOV, Daut; BELUSOUV, Anatolii Sergeevich; VALIDOV, Shamil; YUSUPOV, Marat; USACHEV, Konstantin.},title={Crystal structure of the GDP-bound GTPase era from Staphylococcus aureus},journal={Biochemical and Biophysical Research Communications},note={v. 735, p. 150852-1-150852-7 + supplementary data},year={2024}}