A microbead-enhanced electrochemical platform for ß-amyloid peptide (1-42) detection.
RAZZINO, Claudia do Amaral; SGOBBI, Lívia Flório; BERNARDI, Juliana Cancino; ZAPATA, Angelica Maria Mazuera; COSTA, Clara Cardoso; ZUCOLOTTO, Valtencir; VIEIRA, Lucia; LOBO, Anderson Oliveira.
RAZZINO, Claudia do Amaral; SGOBBI, Lívia Flório; BERNARDI, Juliana Cancino; ZAPATA, Angelica Maria Mazuera; COSTA, Clara Cardoso; ZUCOLOTTO, Valtencir; VIEIRA, Lucia; LOBO, Anderson Oliveira.
Abstract: Alzheimer?s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of ß-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer?s disease is the decreased level of soluble ß-amyloid peptide (1-42) (Aß1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aß1-42 levels drop below 192 pg mL-1. In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aß1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of -200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL-1, with a detection limit of 7.4 pg mL-1, exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer?s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.
@article={003228253,author = {RAZZINO, Claudia do Amaral; SGOBBI, Lívia Flório; BERNARDI, Juliana Cancino; ZAPATA, Angelica Maria Mazuera; COSTA, Clara Cardoso; ZUCOLOTTO, Valtencir; VIEIRA, Lucia; LOBO, Anderson Oliveira.},title={A microbead-enhanced electrochemical platform for ß-amyloid peptide (1-42) detection},journal={Frontiers in Sensors},note={v. 5, 1508810-1-1508810-11, Dec. 2024.},year={2024}}