Antibacterial, cytotoxic and mechanical properties of a orthodontic cement with phosphate nano-sized and phosphorylated chitosan: an in vitro study.
FERNANDES, Gabriela Leal Peres; VANIM, Manuela Marquesini; DELBEM, Alberto Carlos Botazzo; MARTORANO, Antonio Secco; RAUCCI, Larissa Moreira Spinola de Castro; OLIVEIRA, Paulo Tambasco de; ZUCOLOTTO, Valtencir; DIAS, Bruna Juliana Moreira; BRIGHENTI, Fernanda Lourenção; OLIVEIRA, Analú Barros de; MORAES, João Carlos Silos; CAMARGO, Emerson Rodrigues de; DANELON, Marcelle.
FERNANDES, Gabriela Leal Peres; VANIM, Manuela Marquesini; DELBEM, Alberto Carlos Botazzo; MARTORANO, Antonio Secco; RAUCCI, Larissa Moreira Spinola de Castro; OLIVEIRA, Paulo Tambasco de; ZUCOLOTTO, Valtencir; DIAS, Bruna Juliana Moreira; BRIGHENTI, Fernanda Lourenção; OLIVEIRA, Analú Barros de; MORAES, João Carlos Silos; CAMARGO, Emerson Rodrigues de; DANELON, Marcelle.
Abstract: Objectives: Evaluate, in vitro, the effect of incorporating nano-sized sodium trimetaphosphate (TMPnano) and phosphorylated chitosan (Chi-Ph) into resin-modified glass ionomer cement (RMGIC) used for orthodontic bracket cementation, on mechanical, fluoride release, antimicrobial and cytotoxic properties. Methods: RMGIC was combined with Chi-Ph (0.25%/0.5%) and/or TMPnano (14%). The diametral compressive/tensile strength (DCS/TS), surface hardness (SH) and degree of conversion (%DC) were determined. For fluoride (F) release, samples were immersed in des/remineralizing solutions. Antimicrobial/antibiofilm activity was evaluated by the agar diffusion test and biofilm metabolism (XTT). Cytotoxicity in fibroblasts was assessed with the resazurin method. Results: After 24 h, the RMGIC-14%TMPnano group showed a lower TS value (p < 0.001); after 7 days the RMGIC-14%TMPnano-0.25%Chi-Ph group showed the highest value (p < 0.001). For DCS, the RMGIC group (24 h) showed the highest value (p < 0.001); after 7 days, the highest value was observed for the RMGIC-14%TMPnano-0.25%Chi-Ph (p < 0.001). RMGIC-14%TMPnano, RMGIC-14%TMPnano-0.25%Chi-Ph, RMGIC-14%TMPnano-0.5%Chi-Ph showed higher and similar release of F (p > 0.001). In the SH, the RMGIC-0.25%Chi-Ph; RMGIC-0.5%Chi-Ph; RMGIC-14%TMPnano-0.5%Chi-Ph groups showed similar results after 7 days (p > 0.001). The RMGIC-14%TMPnano-0.25%Chi-Ph group showed a better effect on microbial/antibiofilm growth, and the highest efficacy on cell viability (p < 0.001). After 72 h, only the RMGIC-14%TMPnano-0.25%Chi-Ph group showed cell viability (p < 0.001). Conclusion: The RMGIC-14%TMPnano-0.25%Chi-Ph did not alter the physical-mechanical properties, was not toxic to fibroblasts and reduced the viability and metabolism of
@article={003197957,author = {FERNANDES, Gabriela Leal Peres; VANIM, Manuela Marquesini; DELBEM, Alberto Carlos Botazzo; MARTORANO, Antonio Secco; RAUCCI, Larissa Moreira Spinola de Castro; OLIVEIRA, Paulo Tambasco de; ZUCOLOTTO, Valtencir; DIAS, Bruna Juliana Moreira; BRIGHENTI, Fernanda Lourenção; OLIVEIRA, Analú Barros de; MORAES, João Carlos Silos; CAMARGO, Emerson Rodrigues de; DANELON, Marcelle.},title={Antibacterial, cytotoxic and mechanical properties of a orthodontic cement with phosphate nano-sized and phosphorylated chitosan: an in vitro study},journal={Journal of Dentistry},note={v. 146, 105073-1-105073-11, July 2024.},year={2024}}