Recombinant cellobiose dehydrogenase from thermothelomyces thermophilus: its functional characterization and applicability in cellobionic acid production.
OLIVA, Bianca; MENDOZA, Josman Andrey Velasco; BERTO, Gabriela Leila; POLIKARPOV, Igor; OLIVEIRA, Leandro Cristante de; SEGATO, Fernando.
OLIVA, Bianca; MENDOZA, Josman Andrey Velasco; BERTO, Gabriela Leila; POLIKARPOV, Igor; OLIVEIRA, Leandro Cristante de; SEGATO, Fernando.
Abstract: The fungus Thermothelomyces thermophilus is a thermotolerant microorganism that has been explored as a reservoir for enzymes (hydrolytic enzymes and oxidoreductases). The functional analysis of a recombinant cellobiose dehydrogenase (MtCDHB) from T. thermophilus demonstrated a thermophilic behavior, an optimal pH in alkaline conditions for inter-domain electron transfer, and catalytic activity on cellooligosaccharides with different degree of polymerization. Its applicability was evaluated to the sustainable production of cellobionic acid (CBA), a potential pharmaceutical and cosmetic ingredient rarely commercialized. Dissolving pulp was used as a disaccharide source for MtCDHB. Initially, recombinant exoglucanases (MtCBHI and MtCBHII) from T. thermophilus hydrolyzed the dissolving pulp, resulting in 87% cellobiose yield, which was subsequently converted into CBA by MtCDHB, achieving a 66% CBA yield after 24 h. These findings highlight the potential of MtCDHB as a novel approach to obtaining CBA through the bioconversion of a plant-based source.
@article={003194930,author = {OLIVA, Bianca; MENDOZA, Josman Andrey Velasco; BERTO, Gabriela Leila; POLIKARPOV, Igor; OLIVEIRA, Leandro Cristante de; SEGATO, Fernando.},title={Recombinant cellobiose dehydrogenase from thermothelomyces thermophilus: its functional characterization and applicability in cellobionic acid production},journal={Bioresource Technology},note={v. 402, p. 130763-1-130763-11 + supplementary data},year={2024}}