Abstract

3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination.
SUBRAMANIA, Indra Gandi; PERUMAL, Veeradasan; GOPINATH, Subash C. B.; MOHAMED, Norani Muti; JOSHI, Nirav Kumar Jitendrabhai; OVINIS, Mark; SZE, Lim Li.
Abstract: Recently, non-faradaic electrochemical impedimetric (nfEIS) has been identified as a dynamic and robust method for bio-determining. However, the success of a nfEIS approach largely depends on its sensing layer architecture. In this study, the use of a Glutaraldehyde (GA) crosslinked copper (Cu) - bovine serum albumin (BSA) hybrid nanoflower with three-dimensional nanoporous architecture for the sensing layer was investigated. The nanoflower morphology changes were observed under FESEM, revealing loosely interlaced nanoflower into a tightly interlaced, highly porous structure upon GA crosslinking. This nanoflower was hybridized to immobilize aminated-DNA probe on the transducer surface and detect the target TB DNA in their natural redox-free environment. FTIR and XPS characterization showed distinct peaks at 950-1100 cm-1 (P-O, P=O bonds from nanoflower and DNA backbone) and 286.48 eV (interaction between BSA and aminated DNA), respectively, validating the successful DNA probe immobilization on the nanoflower surface. Furthermore, impedimetric sensing in a redox-free environment showed that the developed TB biosensor present has a detection limit (LOD) of 60 pM with a (linear) range from 1 pM to 1 µM with good reproducibility. This redox-free non-faradaic EIS offers excellent biosensing potential and may be extended for diagnosing other biomarkers in clinical practice.
Journal of the Taiwan Institute of Chemical Engineers
v. 116, p. 26-35 - Ano: 2020
Fator de Impacto: 4,794
    @article={003014111,author = {SUBRAMANIA, Indra Gandi; PERUMAL, Veeradasan; GOPINATH, Subash C. B.; MOHAMED, Norani Muti; JOSHI, Nirav Kumar Jitendrabhai; OVINIS, Mark; SZE, Lim Li.},title={3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination},journal={Journal of the Taiwan Institute of Chemical Engineers},note={v. 116, p. 26-35},year={2020}}

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