A multistage formulation based on full-length csp and AMA-1 ectodomain of plasmodium vivax induces high antibody titers and T-cells and partially protects mice challenged with a transgenic plasmodium berghei parasite.
LIMA, Luciana; MARQUES, Rodolfo Ferreira; GIMENEZ, Alba Marina; FRANÇOSO, Katia Sanches; ALIPRANDINI, Eduardo; CAMARGO, Tarsila Mendes de; AGUIAR, Anna Caroline Campos; PEREIRA, Dhelio Batista; RENIA, Laurent; AMINO, Rogerio; SOARES, Irene da Silva.
LIMA, Luciana; MARQUES, Rodolfo Ferreira; GIMENEZ, Alba Marina; FRANÇOSO, Katia Sanches; ALIPRANDINI, Eduardo; CAMARGO, Tarsila Mendes de; AGUIAR, Anna Caroline Campos; PEREIRA, Dhelio Batista; RENIA, Laurent; AMINO, Rogerio; SOARES, Irene da Silva.
Abstract: Infections with Plasmodium vivax are predominant in the Americas, representing 75% of malaria cases. Previously perceived as benign, malaria vivax is, in fact, a highly debilitating and economically important disease. Considering the high complexity of the malaria parasite life cycle, it has been hypothesized that an e ective vaccine formulation against Plasmodium should contain multiple antigens expressed in di erent parasite stages. Based on that, we analyzed a recombinant P. vivax vaccine formulation mixing the apical membrane antigen 1 ectodomain (PvAMA-1) and a full-length circumsporozoite protein (PvCSP-AllFL) previously studied by our group, which elicits a potent antibody response in mice. Genetically distinct strains of mice (C57BL/6 and BALB/c) were immunized with the proteins, alone or in combination, in the presence of poly(I:C) adjuvant, a TLR3 agonist. In C57BL/6, high-antibody titers were induced against PvAMA-1 and the three PvCSP variants (VK210, VK247, and P. vivax-like). Meanwhile, mixing PvAMA-1 with PvCSP-AllFL had no impact on total IgG antibody titers, which were long-lasting. Moreover, antibodies from immunized mice recognized VK210 sporozoites and blood-stage parasites by immunofluorescence assay. However, in the BALB/c model, the antibody response against PvCSP-AllFL was relatively low. PvAMA-1-specific CD3+CD4+ and CD3+CD8+ T-cell responses were observed in C57BL/6 mice, and the cellular response was impaired by PvCSP-AllFL combination. More relevant, the multistage vaccine formulation provided partial protection in mice challenged with a transgenic Plasmodium berghei sporozoite expressing the homologous PvCSP protein.
@article={002998977,author = {LIMA, Luciana; MARQUES, Rodolfo Ferreira; GIMENEZ, Alba Marina; FRANÇOSO, Katia Sanches; ALIPRANDINI, Eduardo; CAMARGO, Tarsila Mendes de; AGUIAR, Anna Caroline Campos; PEREIRA, Dhelio Batista; RENIA, Laurent; AMINO, Rogerio; SOARES, Irene da Silva.},title={A multistage formulation based on full-length csp and AMA-1 ectodomain of plasmodium vivax induces high antibody titers and T-cells and partially protects mice challenged with a transgenic plasmodium berghei parasite},journal={Microorganisms},note={v. 8, n. 6, p. 916-1-916-17},year={2020}}