Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms.
NIE, Min; COSTA E SILVA, Rodrigo; OLIVEIRA, Kleber Thiago de; BAGNATO, Vanderlei Salvador; RASTELLI, Alessandra Nara de Souza; CRIELAARD, Wim; YANG, Jingmei; DENG, Dong Mei.
NIE, Min; COSTA E SILVA, Rodrigo; OLIVEIRA, Kleber Thiago de; BAGNATO, Vanderlei Salvador; RASTELLI, Alessandra Nara de Souza; CRIELAARD, Wim; YANG, Jingmei; DENG, Dong Mei.
Abstract: Antimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 mM), H2O2 (0.3, 3.3, 33.3mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2) 1 (k¼450nm or 660nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment.
@article={003038668,author = {NIE, Min; COSTA E SILVA, Rodrigo; OLIVEIRA, Kleber Thiago de; BAGNATO, Vanderlei Salvador; RASTELLI, Alessandra Nara de Souza; CRIELAARD, Wim; YANG, Jingmei; DENG, Dong Mei.},title={Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms},journal={Biofouling},note={v. 37, n. 6, p. 656-665},year={2021}}