Daptomycin and vancomycin heteroresistance revealed among CC5-SCCmecII MRSA clone and in vitro evaluation of treatment alternatives.
OKADO, Jessica Baleiro; AVACA-CRUSCA, Juliana Sposto; OLIVEIRA, Alexandre Lima; DABUL, Andrei Nicoli Gebieluca; CAMARGO, Ilana Lopes Baratella da Cunha.
OKADO, Jessica Baleiro; AVACA-CRUSCA, Juliana Sposto; OLIVEIRA, Alexandre Lima; DABUL, Andrei Nicoli Gebieluca; CAMARGO, Ilana Lopes Baratella da Cunha.
 Abstract: Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a threat to the success of clinical treatment. Besides high antimicrobial resistance rates, the presence of heterogeneous vancomycin-intermediate S. aureus (hVISA) and heterogeneous daptomycin-non-susceptible S. aureus (hDNSSA) in the hospital environment is underestimated and is associated with treatment failure. The aim of this study was to investigate MRSA dissemination in a Brazilian hospital and to evaluate the efficacy of various treatment options in vitro. Methods MRSA strains were typed by MLST, PFGE and SCCmec typing. Minimum inhibitory concentrations (MICs) to daptomycin, linezolid, quinupristin/dalfopristin, teicoplanin, tetracycline, tigecycline, vancomycin and tedizolid were determined by broth microdilution. The presence of a heterogeneous population was detected by population analysis profile (PAP). Regarding hVISA and hDNSSA strains, the sequences and expression levels of genes involved in resistance to daptomycin and vancomycin were determined as well as cell wall thickness and autolysis. Results: ST5/ST105-SCCmecII lineage was prevalent amongst 27 clinical MRSA characterised in this study. Two hDNSSA strains (one also hVISA) were detected and were confirmed by PAP. Isolate SCMSC29 (hVISA and hDNSSA) showed increased expression of genes involved in cell wall metabolism, slight cell wall thickening, reduction of autolysis, and single nucleotide polymorphisms (SNPs) in the rpoB and mprF genes compared with the susceptible strain SCMSC31. SCMSC35 (hDNSSA) presented SNPs in the rpoB and mprF genes as well as a thickened cell wall. Conclusions Despite this worrying and hard to detect phenotype, treatment alternatives such as teicoplanin, linezolid, tetracycline, tigecycline, quinupristin/da | |
| Journal of Global Antimicrobial Resistance |
| v. 14, p. 209-216 - Ano: 2018 |
| Fator de Impacto: 2,022 |
| http://dx.doi.org/10.1016/j.jgar.2018.05.001 |  @article={002897907,author = {OKADO, Jessica Baleiro; AVACA-CRUSCA, Juliana Sposto; OLIVEIRA, Alexandre Lima; DABUL, Andrei Nicoli Gebieluca; CAMARGO, Ilana Lopes Baratella da Cunha.},title={Daptomycin and vancomycin heteroresistance revealed among CC5-SCCmecII MRSA clone and in vitro evaluation of treatment alternatives},journal={Journal of Global Antimicrobial Resistance},note={v. 14, p. 209-216},year={2018}} |