Kidney decontamination during perfusion for transplantation procedure: in vitro and ex vivo viability analysis.
MAFUD, Loraine Carolina Goenaga; GÁMEZ, Yordania Matos; CAMPOS, Carolina de Paula; VOLLET FILHO, José Dirceu; INADA, Natalia Mayumi; KURACHI, Cristina; BAGNATO, Vanderlei Salvador.
MAFUD, Loraine Carolina Goenaga; GÁMEZ, Yordania Matos; CAMPOS, Carolina de Paula; VOLLET FILHO, José Dirceu; INADA, Natalia Mayumi; KURACHI, Cristina; BAGNATO, Vanderlei Salvador.
Abstract: Organ transplantations have an increasing medical relevance. It is becoming a regular procedure with an increase in individuals waiting for organs. The increase in the number of discarded organs is mostly due to the donor bacterial and/or viral infection. In this article, we are demonstrating the feasibility of reduction of the bacterial load in kidney model by using ultraviolet-C as a germicidal agent in circulating liquids. Using Staphylococcus aureus as a bacteria model, we were able to demonstrate that in less than 30 min of liquid circulation and associated to irradiation, the bacterial load of the perfusate Custodiol HTK, histidine-tryptophan-ketoglutarate (solution with 5 log CFU mL 1), was fully eliminated. A modeling approach was created to verify the possibility of bacterial load decrease, when an organ (here, a renal experimental model) is present in the circuit, releasing a varied rate of microorganisms over time, while the solution is irradiated. Finally, we use an ex vivo model with swine kidney, circulating in the preservation solution with a Lifeport Kidney Transporter machine, to demonstrate that we can contaminate the organ and then promote the elimination of the microbiological load. The results show the feasibility of the technique.
@article={003074561,author = {MAFUD, Loraine Carolina Goenaga; GÁMEZ, Yordania Matos; CAMPOS, Carolina de Paula; VOLLET FILHO, José Dirceu; INADA, Natalia Mayumi; KURACHI, Cristina; BAGNATO, Vanderlei Salvador.},title={Kidney decontamination during perfusion for transplantation procedure: in vitro and ex vivo viability analysis},journal={Journal of Biophotonics},note={v. 15, n. 5, p. e202100319-1-e202100319-11},year={2022}}