Abstract – Publication

Quantification of paramagnetic ions in human brain tissue using EPR.
OTSUKA, Fábio Seiji; OTADUY, Maria Concepción García; NASCIMENTO, Otaciro Rangel; SALMON, Carlos Ernesto Garrido.
Abstract: The content and molecular state of paramagnetic ions were assessed by electron paramagnetic resonance (EPR) spectroscopy in ex vivo human brain samples from the globus pallidus, locus coeruleus, and substantia nigra. A dedicated pipeline of quantitative deconvolution and simulation-informed spectral fitting was employed to resolve five overlapping resonance peaks in the EPR signal present in the (whole tissue) brain samples. Simulated results showed good visual agreement with experimental spectral signals. Our quantitative analytical pipeline enabled theorizing understanding of the molecular roles of Iron and other ions in the evaluated specialized subcortical regions. Analysis of the absorption and linewidth of each peak-studied as a function of temperature-showed that four of these peaks behave like a Curie-Weiss paramagnet with an antiferromagnetic contribution, while a broader peak (centered at 320 mT) showed a strong antiferromagnetic behavior with ferro/ferrimagnetic contribution. Noticeably, a fifth peak, which shifts from 190 to 230 mT as temperature increases, was observed exclusively in the locus coeruleus region and can be specifically associated with a possible iron complex formed during the oxidation of dopamine. Our findings support the translational aspects of EPR to inform the cellular-molecular nature of Iron loads phenotypically visible in associated magnetic resonance susceptibility and relaxometry imaging techniques, that are broadly applied to the radiologic characterization of a plethora of neurologic disorders.
Brazilian Journal of Physics
v. 52, n. 3, p. 94-1-94-10 - Ano: 2022
Fator de Impacto: 1,326
    @article={003072981,author = {OTSUKA, Fábio Seiji; OTADUY, Maria Concepción García; NASCIMENTO, Otaciro Rangel; SALMON, Carlos Ernesto Garrido.},title={Quantification of paramagnetic ions in human brain tissue using EPR},journal={Brazilian Journal of Physics},note={v. 52, n. 3, p. 94-1-94-10},year={2022}}

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