Abstract – Publication

Cancer immunosensor based on apo and holo transferrin binding.
ALMEIDA, Sthéfane Valle de; CANCINO-BERNARDI, Juliana; ANDRADE, Jucimara Kulek de; FELSNER, Maria Lurdes; ZUCOLOTTO, Valtencir; GALLI, Andressa.
Abstract: An electrochemical immunosensor was developed for the determination of apo-Tf (non-iron-bound) and holo-Tf (iron-bound) using polyclonal antibody transferrin (anti-Tf) immobilized at an electrode surface as a biorecognition platform. The monitoring was based on the anti-Tf binding with both Tf forms which allows the detection of cancer cells due to the constant iron cycle and the overexpression of anti-Tf on the cancer cell surface. The immunosensor characterization was performed using electrochemical impedance spectroscopy (EIS), which evaluated the impedimetric biorecognition of the antigens-antibody by the use of K4Fe(CN)6 redox group. The immunosensor was able to detect both forms of Tf in terms of charge transfer resistance (Rct). Analytical curves showed a limit of detection of 0.049 and 0.053 ng mL-1 for apo-Tf and holo-Tf, respectively. The immunosensor was applied to the detection of the two cancer cells A549 (lung carcinoma) andMCF-7 (breast carcinoma) and compared with BHK570, a healthy cell line. The impedimetric response of healthy cells differs significantly from that of the cancerous cells, as revealed by a Dunnett?s test in 95% confidence level- ca. 102 cells mL-1-indicating the feasibility of the immunosensor to discriminate both types of cells. The indirect detection of anti-Tf based on apo-Tf and holo-Tf binding can be considered an advanced approach for cancer recognition.
Microchimica Acta
v. 187, n. 8, p. 438-1-438-9 - Ano: 2020
Fator de Impacto: 6,232
    @article={003000480,author = {ALMEIDA, Sthéfane Valle de; CANCINO-BERNARDI, Juliana; ANDRADE, Jucimara Kulek de; FELSNER, Maria Lurdes; ZUCOLOTTO, Valtencir; GALLI, Andressa.},title={Cancer immunosensor based on apo and holo transferrin binding},journal={Microchimica Acta},note={v. 187, n. 8, p. 438-1-438-9},year={2020}}

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