Abstract – Publication

Immunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect D-dimer as biomarker for venous thromboembolism.
RODRIGUES, Valquiria C.; MORAES, Marli L.; SOARES, Juliana C.; SOARES, Andrey C.; SANFELICE, Rafaela; DEFFUNE, Elenice; OLIVEIRA JÚNIOR, Osvaldo Novais de.
Abstract: We report on immunosensors to detect D-dimer, a biomarker of venous thromboembolism, which are made with layer-by-layer (LbL) films containing immobilized anti-D-dimer monoclonal antibody alternated with a layer of chitosan/gold nanoparticles (AuNpChi). Detection was due to irreversible adsorption of the antigen D-dimer on its corresponding antibody according to a Langmuir-Freundlich model, thus giving rise to ellipsoidal structures in scanning electron microscopy images whose size and number increased with D-dimer concentration. The chemical groups involved in the adsorption process were inferred from polarization-modulated infrared reflection absorption (PM-IRRAS) through changes in the amide and carbonyl bands. Detection of D-dimer was made with electrical impedance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The latter was the most sensitive with a detection limit of 9 × 10-4 µg/mL, sensitivity of 0.27 × 10-6 A/µgmL-1 with linear increase from 0 to 1 µg/mL. The selectivity of the immunosensor made with AuNpChi/anti-D-dimer film was verified by noting negligible changes in the cyclic voltammograms when exposed to typical interferents in biological fluids.
Bulletin of the Chemical Society of Japan
v. 91, n. 6, p. 891-896 - Ano: 2018
Fator de Impacto: 2,297
http://dx.doi.org/10.1246/bcsj.20180019
    @article={002886970,author = {RODRIGUES, Valquiria C.; MORAES, Marli L.; SOARES, Juliana C.; SOARES, Andrey C.; SANFELICE, Rafaela; DEFFUNE, Elenice; OLIVEIRA JÚNIOR, Osvaldo Novais de.},title={Immunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect D-dimer as biomarker for venous thromboembolism},journal={Bulletin of the Chemical Society of Japan},note={v. 91, n. 6, p. 891-896},year={2018}}

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