Immunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect D-dimer as biomarker for venous thromboembolism.
RODRIGUES, Valquiria C.; MORAES, Marli L.; SOARES, Juliana C.; SOARES, Andrey C.; SANFELICE, Rafaela; DEFFUNE, Elenice; OLIVEIRA JÚNIOR, Osvaldo Novais de.
RODRIGUES, Valquiria C.; MORAES, Marli L.; SOARES, Juliana C.; SOARES, Andrey C.; SANFELICE, Rafaela; DEFFUNE, Elenice; OLIVEIRA JÚNIOR, Osvaldo Novais de.
Abstract: We report on immunosensors to detect D-dimer, a biomarker of venous thromboembolism, which are made with layer-by-layer (LbL) films containing immobilized anti-D-dimer monoclonal antibody alternated with a layer of chitosan/gold nanoparticles (AuNpChi). Detection was due to irreversible adsorption of the antigen D-dimer on its corresponding antibody according to a Langmuir-Freundlich model, thus giving rise to ellipsoidal structures in scanning electron microscopy images whose size and number increased with D-dimer concentration. The chemical groups involved in the adsorption process were inferred from polarization-modulated infrared reflection absorption (PM-IRRAS) through changes in the amide and carbonyl bands. Detection of D-dimer was made with electrical impedance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The latter was the most sensitive with a detection limit of 9 × 10-4 µg/mL, sensitivity of 0.27 × 10-6 A/µgmL-1 with linear increase from 0 to 1 µg/mL. The selectivity of the immunosensor made with AuNpChi/anti-D-dimer film was verified by noting negligible changes in the cyclic voltammograms when exposed to typical interferents in biological fluids. | |
Bulletin of the Chemical Society of Japan |
v. 91, n. 6, p. 891-896 - Ano: 2018 |
Fator de Impacto: 2,297 |
http://dx.doi.org/10.1246/bcsj.20180019 | @article={002886970,author = {RODRIGUES, Valquiria C.; MORAES, Marli L.; SOARES, Juliana C.; SOARES, Andrey C.; SANFELICE, Rafaela; DEFFUNE, Elenice; OLIVEIRA JÚNIOR, Osvaldo Novais de.},title={Immunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect D-dimer as biomarker for venous thromboembolism},journal={Bulletin of the Chemical Society of Japan},note={v. 91, n. 6, p. 891-896},year={2018}} |