An insight into molecular motions and phase composition of gliadin/glutenin glycerol blends studied by 13C solid-state and 1H time-domain NMR.
ANDRADE, Fabiana Diuk; NEWSON, William R.; BERNARDINELLI, Oigres Daniel; RASHEED, Faiza; COBO, Márcio Fernando; PLIVELIC, Tomás S.; AZEVÊDO, Eduardo Ribeiro de; KUKTAITE, Ramune.
ANDRADE, Fabiana Diuk; NEWSON, William R.; BERNARDINELLI, Oigres Daniel; RASHEED, Faiza; COBO, Márcio Fernando; PLIVELIC, Tomás S.; AZEVÊDO, Eduardo Ribeiro de; KUKTAITE, Ramune.
 Abstract: Monitoring of the molecular motions and secondary structures of gliadin (Gli) and glutenin (Glu) in blends with 10, 20, 30, and 40% glycerol was performed by solid-state (SS) and time domain (TD) NMR spectroscopy. Increasing the glycerol content increased the relative amount of b-sheets and disordered structures, while decreasing a-helices in Gli/Glu-glycerol blends studied by 13C CPMAS NMR. For 20% glycerol samples, the protein side-chain mobility increased similarly for Gli and Glu. A higher proportion of a-helices versus b-sheets was found in Gli-glycerol blends compared with Glu-glycerol blends.Glycerol acted as ?immobilized? in 10-20% glycerol Gli samples and was found mainly ?free? in 30 and 40% glycerol Gli/Glu samples. During temperature experiments, 30 and 40% glycerol amounts impacted the dynamic molecular behavior of the Gli and Glu proteins differently than lipids, as observed by TDNMR. The combination of TD-NMR together with SS-NMR showed details of the dynamic molecular variations in Gli/Glu protein structure and are promising techniques to monitor the molecular dynamics of plasticized proteins. | |
| Journal of Polymer Science B |
| v. 56, n. 9, p. 739-750 - Ano: 2018 |
| Fator de Impacto: 2,838 |
| http://dx.doi.org/0.1002/polb.24586 |  @article={002878500,author = {ANDRADE, Fabiana Diuk; NEWSON, William R.; BERNARDINELLI, Oigres Daniel; RASHEED, Faiza; COBO, Márcio Fernando; PLIVELIC, Tomás S.; AZEVÊDO, Eduardo Ribeiro de; KUKTAITE, Ramune.},title={An insight into molecular motions and phase composition of gliadin/glutenin glycerol blends studied by 13C solid-state and 1H time-domain NMR},journal={Journal of Polymer Science B},note={v. 56, n. 9, p. 739-750},year={2018}} |
